plant tissue culture techniques were used to study the effect of nacl on tropane alkaloids accumulation and proline content of both datura metel and datura stramonium. callus cultures were established for both datura species. however, ms medium supplemented with 1mg/l of both ba and naa was the best for callus production in d. metel, while ms supplemented with 3 mg/l kin and 1 mg/l 2, 4-d was t...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a potential risk of toxicity for humans and animals. Therefore, a reliable method for the determination of tropane alkaloid content is of high imp...

A forensic method comprising solid phase extraction and HPLC analysis was developed for the detection and confirmation of atropine and scopolamine, the main toxic alkaloids of Datura stramonium and Datura ferox. This method allowed the direct coupling of an electrospray (ZMD) mass selective detector to the HPLC system. Under these conditions, atropine and scopolamine were well separated from ot...

The purpose of this study was to determine the atropine and scopolamine content in different parts of Datura plant, (Datura metel L.), growing wild in Syria. These alkaloids were separated and quantified by high-performance liquid chromatography (HPLC) coupled with diode-array detector (DAD), and fluorescence detector (FLD), using a reversed phase Eclipse C18 column and employing a mixture of w...

The ethanol extracts of Datura stramonium and Datura metel (Family: Solanaceae) collected from Pappakovil, Nagapattinam Dist of Tamil Nadu, India. The Ethanol extract obtained by maceration extraction was examined for its antioxidant activities. The antioxidant activity was determined by means of the DPPH radical scavenging test, Total antioxidant (TAA) and superoxide anion scavenging activity ...

Datura stramonium (DS) is a widespread annual plant, containing atropine, hyoscyamine, and scopolamine, which can produce poisoning with a severe anticholinergic syndrome. Teenagers ingest the roots, seeds or the entire plant to obtain its hallucinogenic and euphoric effects. We presented the case of a 22 year old male who was admitted to the Emergency Room in a coma after consuming Datura stra...

In the early morning hours of July 9, 2008, six adult family members were admitted to a hospital emergency department in Maryland with hallucinations, confusion, mydriasis, and tachycardia of approximately 3-4 hours duration. Approximately 4-5 hours earlier, all six family members had shared a meal of homemade stew and bread. Subsequent investigation by the Montgomery County Department of Healt...